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Furthermore, the biosurfactant produced was found to belong to the class, phospholipids based on the TLC and GC–MS analyses.Microorganisms that produce biosurfactant abound in nature; they inhabit both water (fresh water, groundwater, and sea) and land (soil, sediment and sludge).
Examples of low molecular weight biosurfactants are the glycolipids, lipopeptides and lipoprotein, fatty acids, phospholipids, neutral lipids, particulate biosurfactants, and polymeric biosurfactant while the high molecular weight biosurfactants are composed of polysaccharides, proteins, lipopolysaccharides, lipoproteins, or complex mixtures of these biopolymers.
The best studied bioemulsifiers are the bioemulsans produced by different species of Acinetobacter (Rosenberg and Ron 1998).
Thin-layer chromatography (TLC) and gas chromatography mass spectrometry (GC–MS) analyses were used in the classification and characterization of the biosurfactant produced.
The biosurfactant produced was applied on selected hydrocarbons to determine its emulsifying capacity.
In addition, the biosurfactant showed emulsifying activity against the following hydrocarbons: petrol, kerosene, xylene, toluene, and diesel.
The optimum cultural conditions (temperature, p H, carbon, nitrogen, hydrocarbon, inoculum concentration, and incubation time) for growth and biosurfactant production by K. The biosurfactant was characterized as a phospholipid using TLC, while the GC–MS analysis identified the phospholipid as phosphatidylethanolamine. pneumoniae strain IVN51 isolated from hydrocarbon-polluted soil to produce biosurfactant and the effectiveness of the produced biosurfactant in emulsifying different hydrocarbons.
This study investigated the isolation, characterization, and application in hydrocarbon emulsification of biosurfactant by Klebsiella pneumoniae strain IVN51 isolated from hydrocarbon-contaminated soil in Ogoniland, Nigeria.
The soil samples used for bacterial isolation were obtained from the Kporghor community of Tai Local Government Area (Ogoniland), in the Niger Delta region of Nigeria.
For each soil source, soil samples were randomly collected from different points at depths between 0 and 15 cm using a hand-held soil auger and then bulked to get a composite sample.
The samples were transported aseptically in sterile polythene bags to the laboratory for the analysis.